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PRPH ELISA Kit (Human)

CAT: 0247-OKEH03505-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03505-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Peripherin
Gene Aliases
NEF4; Neurofilament 4; neurofilament 4 (57kD) ; peripherin; PRPH1.
Gene ID
5630
Accession Number
NP_006253.2
Reactivity
Homo sapiens, Human
Target
Peripherin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology PRPH ELISA Kit (Human) (OKEH03505) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PRPH antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PRPH and incubated. The PRPH found in the sample or standards competes with the biotinylated PRPH for limited binding sites on the immobilized anti-human PRPH antibody. Excess unbound biotinylated PRPH and sample or standard PRPH is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PRPH captured in the well and inversely proportional to the amount of PRPH which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
54 kDa
Specificity
Natural and recombinant Human Peripherin
Protein Length
470
NCBI Gene Symbol
PRPH
Protein Name
P41219
Gene Name URL
PRPH
Nucleotide Accession Number
NM_006262.3

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