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LOX ELISA Kit (Rat)

CAT: 0247-OKEH03492-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03492-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Lysyl oxidase
Gene Aliases
H-rev142; Lysyl oxidase; Lysyl oxidase (an H-rev gene with its expression down-regulated in HRAS-transformed rat 208F fibroblasts) ; protein-lysine 6-oxidase; Rrg1.
Gene ID
24914
Accession Number
NP_058757.1
Reactivity
Rat, Rattus norvegicus
Target
Protein-lysine 6-oxidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LOX ELISA Kit (Rat) (OKEH03492) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lox has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Lox is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lox captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Sensitivity
39.7 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Specificity
Natural and recombinant Rat Protein-lysine 6-oxidase
Protein Length
411
NCBI Gene Symbol
Lox
Protein Name
P16636
Gene Name URL
Lox
Nucleotide Accession Number
NM_017061.2

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