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CAPN2 ELISA Kit (Rat)

CAT: 0247-OKEH03488-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03488-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Calpain 2
Gene Aliases
Calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain II; calpain M-type; calpain-2 catalytic subunit; calpain-2 large subunit; CANP 2; M-calpain; millimolar-calpain.
Gene ID
29154
Accession Number
NP_058812.1
Reactivity
Rat, Rattus norvegicus
Target
Calpain-2 catalytic subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CAPN2 ELISA Kit (Rat) (OKEH03488) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Capn2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Capn2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Capn2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.16 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
79 kDa
Specificity
Natural and recombinant Rat Calpain-2 catalytic subunit
Protein Length
700
NCBI Gene Symbol
Capn2
Protein Name
Q07009
Gene Name URL
Capn2
Nucleotide Accession Number
NM_017116.2

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