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ADAMTS5 ELISA Kit (Mouse)

CAT: 0247-OKEH03453-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03453-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Gene Aliases
9530092O11Rik; A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-; ADAMTS1; ADAMTS11; ADAM-TS5; ADMP-2; aggrecanase-2; AI481094; ASMP-2; implantin.
Gene ID
23794
Accession Number
NP_035912.2
Reactivity
Mouse, Mus musculus
Target
A disintegrin and metalloproteinase with thrombospondin motifs 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ADAMTS5 ELISA Kit (Mouse) (OKEH03453) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adamts5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adamts5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adamts5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Sensitivity
7.98 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
102 kDa
Specificity
Natural and recombinant Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5
Protein Length
930
NCBI Gene Symbol
Adamts5
Protein Name
Q9R001
Gene Name URL
Adamts5
Nucleotide Accession Number
NM_011782.2

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