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PRKAB1 ELISA Kit (Rat)

CAT: 0247-OKEH03395-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03395-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Protein kinase AMP-activated non-catalytic subunit beta 1
Gene Aliases
5'-AMP-activated protein kinase 40 kDa subunit;5-AMP-activated protein kinase beta subunit;5'-AMP-activated protein kinase subunit beta-1;5'-AMP-activated protein kinase, beta subunit; AMPK beta-1 chain; AMPK subunit beta-1; AMPKb; protein kinase, AMP-activated, beta 1 non-catalytic subunit.
Gene ID
83803
Accession Number
NP_114182.1
Reactivity
Rat, Rattus norvegicus
Target
5'-AMP-activated protein kinase subunit beta-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRKAB1 ELISA Kit (Rat) (OKEH03395) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prkab1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prkab1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prkab1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
30 kDa
Specificity
Natural and recombinant Rat 5'-AMP-activated protein kinase subunit beta-1
Protein Length
270
NCBI Gene Symbol
Prkab1
Protein Name
P80386
Gene Name URL
Prkab1
Nucleotide Accession Number
NM_031976.1

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