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HMGB2 ELISA Kit (Mouse)

CAT: 0247-OKEH03371-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03371-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
High mobility group box 2
Gene Aliases
C80539; high mobility group protein 2; high mobility group protein B2; Hmg2; HMG-2.
Gene ID
97165
Accession Number
NP_032278.1
Reactivity
Mouse, Mus musculus
Target
High mobility group protein B2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HMGB2 ELISA Kit (Mouse) (OKEH03371) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hmgb2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hmgb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hmgb2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
24 kDa
Specificity
Natural and recombinant Mouse High mobility group protein B2
Protein Length
210
NCBI Gene Symbol
Hmgb2
Protein Name
P30681
Gene Name URL
Hmgb2
Nucleotide Accession Number
NM_008252.3

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