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AKTIP ELISA Kit (Human)

CAT: 0247-OKEH03345-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03345-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
AKT interacting protein
Gene Aliases
AKT-interacting protein; FT1; FTS; fused toes homolog; Fused toes protein homolog.
Gene ID
64400
Accession Number
NP_001012398.1
Reactivity
Homo sapiens, Human
Target
AKT-interacting protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AKTIP ELISA Kit (Human) (OKEH03345) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKTIP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for AKTIP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKTIP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
33 kDa
Specificity
Natural and recombinant Human AKT-interacting protein
Protein Length
292
NCBI Gene Symbol
AKTIP
Protein Name
Q9H8T0
Gene Name URL
AKTIP
Nucleotide Accession Number
NM_001012398.2

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