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CMKLR1 ELISA Kit (Human)

CAT: 0247-OKEH03335-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03335-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Chemerin chemokine-like receptor 1
Gene Aliases
Chemerin receptor; CHEMERINR; chemokine receptor-like 1; chemokine-like receptor 1; ChemR23; DEZ; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor, Dez; resolvin E1 receptor; RVER1.
Gene ID
1240
Accession Number
NP_001135815.1
Reactivity
Homo sapiens, Human
Target
Chemokine-like receptor 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CMKLR1 ELISA Kit (Human) (OKEH03335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CMKLR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CMKLR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CMKLR1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
39.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Chemokine-like receptor 1
Protein Length
373
NCBI Gene Symbol
CMKLR1
Protein Name
Q99788
Gene Name URL
CMKLR1
Nucleotide Accession Number
NM_001142343.1