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LCORL ELISA Kit (Human)

CAT: 0247-OKEH03326-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03326-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Ligand dependent nuclear receptor corepressor like
Gene Aliases
LCOR-like protein; ligand-dependent nuclear receptor corepressor-like protein; MBLK1-related protein; MLR1; transcription factor MLR1.
Gene ID
254251
Accession Number
NP_001159611.1
Reactivity
Homo sapiens, Human
Target
Ligand-dependent nuclear receptor corepressor-like protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology LCORL ELISA Kit (Human) (OKEH03326) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LCORL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated LCORL and incubated. The LCORL found in the sample or standards competes with the biotinylated LCORL for limited binding sites on the immobilized anti-Human LCORL antibody. Excess unbound biotinylated LCORL and sample or standard LCORL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated LCORL captured in the well and inversely proportional to the amount of LCORL which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87.9%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Specificity
Natural and recombinant Human Ligand-dependent nuclear receptor corepressor-like protein
Protein Length
602
NCBI Gene Symbol
LCORL
Protein Name
Q8N3X6
Gene Name URL
LCORL
Nucleotide Accession Number
NM_001166139.1

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