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TAC3 ELISA Kit (Rat)

CAT: 0247-OKEH03314-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03314-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tachykinin precursor 3
Gene Aliases
Neurokinin B; Tac2; tachykinin 2; tachykinin 3; tachykinin-3.
Gene ID
29191
Accession Number
NP_062035.1
Reactivity
Rat, Rattus norvegicus
Target
Tachykinin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TAC3 ELISA Kit (Rat) (OKEH03314) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Tac3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tac3 and incubated. The Tac3 found in the sample or standards competes with the biotinylated Tac3 for limited binding sites on the immobilized anti-Rat Tac3 antibody. Excess unbound biotinylated Tac3 and sample or standard Tac3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tac3 captured in the well and inversely proportional to the amount of Tac3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
41.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Rat Tachykinin-3
Protein Length
116
NCBI Gene Symbol
Tac3
Protein Name
P08435
Gene Name URL
Tac3
Nucleotide Accession Number
NM_019162.2

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