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RNLS ELISA Kit (Rat)

CAT: 0247-OKEH03288-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03288-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Renalase, FAD-dependent amine oxidase
Gene Aliases
Alpha-NAD (P) H oxidase/anomerase; MAO-C; monoamine oxidase-C; renalase; RGD1309804.
Gene ID
361751
Accession Number
NP_001014189.1
Reactivity
Rat, Rattus norvegicus
Target
Renalase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RNLS ELISA Kit (Rat) (OKEH03288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rnls has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Rnls is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rnls captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into plasma : 104%
Sensitivity
1.79 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 kDa
Specificity
Natural and recombinant Rat Renalase
Protein Length
315
NCBI Gene Symbol
Rnls
Protein Name
Q5U2W9
Gene Name URL
Rnls
Nucleotide Accession Number
NM_001014167.1

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