ABCC1 ELISA Kit (Mouse)

CAT: 0247-OKEH03280-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03280-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Gene Aliases
Abcc; Abcc1a; Abcc1b; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1a; ATP-binding cassette, sub-family C (CFTR/MRP), member 1b; glutathione-S-conjugate-translocating ATPase ABCC1; leukotriene C (4) transporter; LTC4 transporter; M; Md; Mdrap; Mr; MRP; Mrp1; multidrug resistance-associated protein 1; multiple drug resistance-associated protein.
Gene ID
17250
Accession Number
NP_032602.1
Reactivity
Mouse, Mus musculus
Target
Multidrug resistance-associated protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ABCC1 ELISA Kit (Mouse) (OKEH03280) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abcc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Abcc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abcc1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
171 kDa
Specificity
Natural and recombinant Mouse Multidrug resistance-associated protein 1
Protein Length
1528
NCBI Gene Symbol
Abcc1
Protein Name
O35379
Gene Name URL
Abcc1
Nucleotide Accession Number
NM_008576.3

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