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BECN1 ELISA Kit (Rat)

CAT: 0247-OKEH03267-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03267-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Beclin 1
Gene Aliases
Beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) ; beclin 1, autophagy related; beclin-1; Beclin1; coiled-coil myosin-like BCL2-interacting protein; Protein GT197.
Gene ID
114558
Accession Number
NP_001029289.1
Reactivity
Rat, Rattus norvegicus
Target
Beclin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BECN1 ELISA Kit (Rat) (OKEH03267) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Becn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Becn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Becn1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 kDa
Specificity
Natural and recombinant Rat Beclin-1
Protein Length
448
NCBI Gene Symbol
Becn1
Protein Name
Q91XJ1
Gene Name URL
Becn1
Nucleotide Accession Number
NM_001034117.1

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