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LPAR3 ELISA Kit (Mouse)

CAT: 0247-OKEH03260-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03260-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Lysophosphatidic acid receptor 3
Gene Aliases
Edg7; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor 7; L; LPA receptor 3; lpA3; LPA-3; lysophosphatidic acid receptor 3; lysophosphatidic acid receptor Edg-7.
Gene ID
65086
Accession Number
NP_075359.1
Reactivity
Mouse, Mus musculus
Target
Lysophosphatidic acid receptor 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LPAR3 ELISA Kit (Mouse) (OKEH03260) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lpar3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lpar3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lpar3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
40 kDa
Specificity
Natural and recombinant Mouse Lysophosphatidic acid receptor 3
Protein Length
354
NCBI Gene Symbol
Lpar3
Protein Name
Q9EQ31
Gene Name URL
Lpar3
Nucleotide Accession Number
NM_022983.4

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