HCRT ELISA Kit (Rat)

• Gene Name:

  Hypocretin neuropeptide precursor

• Gene Aliases:

  Hypocretin; Hypocretin (orexin) neuropeptide; orexin; orexin-A.

• Gene ID:

  25723

• Accession Number:

  NP_037311.1

• Reactivity:

  Rat, Rattus norvegicus

• Target:

  Orexin

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology HCRT ELISA Kit (Rat) (OKEH03216) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hcrt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hcrt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hcrt captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

• Detection Range:

  7.8-500pg/mL

• Sensitivity:

  3.9 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  14 kDa

• Specificity:

  Natural and recombinant Rat Orexin

• Protein Length:

  130

• NCBI Gene Symbol:

  Hcrt

• Protein Name:

  O55232

• Gene Name URL:

  Hcrt

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_013179.2