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HIVEP2 ELISA Kit (Human)

CAT: 0247-OKEH03170-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03170-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
HIVEP zinc finger 2
Gene Aliases
C-myc intron binding protein 1; HIV-EP2; human immunodeficiency virus type I enhancer binding protein 2; Human immunodeficiency virus type I enhancer-binding protein 2; MBP-2; MHC binding protein-2; MHC-binding protein 2; MIBP1; MRD43; Schnurri-2; SHN2; transcription factor HIVEP2; ZAS2; ZNF40B.
Gene ID
3097
Accession Number
NP_006725.3
Reactivity
Homo sapiens, Human
Target
Transcription factor HIVEP2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology HIVEP2 ELISA Kit (Human) (OKEH03170) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human HIVEP2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HIVEP2 and incubated. The HIVEP2 found in the sample or standards competes with the biotinylated HIVEP2 for limited binding sites on the immobilized anti-human HIVEP2 antibody. Excess unbound biotinylated HIVEP2 and sample or standard HIVEP2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HIVEP2 captured in the well and inversely proportional to the amount of HIVEP2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
269 kDa
Specificity
Natural and recombinant Human Transcription factor HIVEP2
Protein Length
2446
NCBI Gene Symbol
HIVEP2
Protein Name
P31629
Gene Name URL
HIVEP2
Nucleotide Accession Number
NM_006734.3

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