NPPB ELISA Kit (Mouse)

• Reproducibility :

  Mean Inter-assay CV%:

• Gene Name :

  Natriuretic peptide type B

• Gene Aliases :

  AA408272; B; BNF; BNP; brain natriuretic factor prohormone; brain natriuretic peptide; gamma-brain natriuretic peptide; Iso-ANP; natriuretic peptide precursor type B; natriuretic peptides B; preproBNP; proBNP; type-B natriuretic factor.

• Gene ID :

  18158

• Accession Number :

  NP_001274277.1

• Reactivity :

  Mouse, Mus musculus

• Target :

  Natriuretic peptides B

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle :

  Aviva Systems Biology NPPB ELISA Kit (Mouse) (OKEH03141) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Nppb antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nppb and incubated. The Nppb found in the sample or standards competes with the biotinylated Nppb for limited binding sites on the immobilized anti-mouse Nppb antibody. Excess unbound biotinylated Nppb and sample or standard Nppb is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nppb captured in the well and inversely proportional to the amount of Nppb which was contained in the sample or standard.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

• Detection Range :

  15.6-1000pg/mL

• Recovery :

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

• Sensitivity :

  7.88 pg/mL

• Reconstitution :

  Store as indicated in product manual.

• Molecular Weight :

  14 kDa

• Specificity :

  Natural and recombinant Mouse Natriuretic peptides B

• Protein Length :

  121

• NCBI Gene Symbol :

  Nppb

• Protein Name :

  P40753

• Gene Name URL :

  Nppb

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001287348.1