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ENO2 ELISA Kit (Mouse)

CAT: 0247-OKEH03138-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03138-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Enolase 2, gamma neuronal
Gene Aliases
2-phospho-D-glycerate hydro-lyase; AI837106; D6Ertd375; D6Ertd375e; Eno; Eno-2; Enolase 2; gamma-enolase; N; neural enolase; neuron-specific enolase; NSE.
Gene ID
13807
Accession Number
NP_001289571.1
Reactivity
Mouse, Mus musculus
Target
Gamma-enolase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ENO2 ELISA Kit (Mouse) (OKEH03138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eno2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Eno2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eno2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
0.183 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
47 kDa
Specificity
Natural and recombinant Mouse Gamma-enolase
Protein Length
434
NCBI Gene Symbol
Eno2
Protein Name
P17183
Gene Name URL
Eno2
Nucleotide Accession Number
NM_001302642.1