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PRDX4 ELISA Kit (Rat)

CAT: 0247-OKEH03119-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03119-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Peroxiredoxin 4
Gene Aliases
Antioxidant enzyme AOE372; peroxiredoxin IV; peroxiredoxin-4; prx-IV; thioredoxin peroxidase AO372; thioredoxin-dependent peroxide reductase A0372; thioredoxin-dependent peroxiredoxin 4.
Gene ID
85274
Accession Number
NP_445964.1
Reactivity
Rat, Rattus norvegicus
Target
Peroxiredoxin-4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRDX4 ELISA Kit (Rat) (OKEH03119) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Prdx4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prdx4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Rat Peroxiredoxin-4
Protein Length
273
NCBI Gene Symbol
Prdx4
Protein Name
Q9Z0V5
Gene Name URL
Prdx4
Nucleotide Accession Number
NM_053512.2

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