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S1PR3 ELISA Kit (Mouse)

CAT: 0247-OKEH03089-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03089-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Sphingosine-1-phosphate receptor 3
Gene Aliases
AI132464; Edg3; endothelial differentiation G-protein coupled receptor 3; endothelial differentiation sphingolipid G-protein-coupled receptor 3; LP; Lpb3; lysophospholipid receptor B3; S1; S1p receptor 3; S1P receptor Edg-3; S1p3; sphingosine 1-phosphate receptor 3; sphingosine 1-phosphate receptor Edg-3.
Gene ID
13610
Accession Number
NP_034231.1
Reactivity
Mouse, Mus musculus
Target
Sphingosine 1-phosphate receptor 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology S1PR3 ELISA Kit (Mouse) (OKEH03089) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse S1pr3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated S1pr3 and incubated. The S1pr3 found in the sample or standards competes with the biotinylated S1pr3 for limited binding sites on the immobilized anti-mouse S1pr3 antibody. Excess unbound biotinylated S1pr3 and sample or standard S1pr3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated S1pr3 captured in the well and inversely proportional to the amount of S1pr3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.158 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Mouse Sphingosine 1-phosphate receptor 3
Protein Length
378
NCBI Gene Symbol
S1pr3
Protein Name
Q9Z0U9
Gene Name URL
S1pr3
Nucleotide Accession Number
NM_010101.4

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