SLC8A1 ELISA Kit (Mouse)

CAT: 0247-OKEH03086-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03086-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Solute carrier family 8 (sodium/calcium exchanger), member 1
Gene Aliases
AI852629; AV344025; D930008O12Rik; Na (+) /Ca (2+) -exchange protein 1; Na/Ca exchanger; Ncx; Ncx1; sodium/calcium exchanger 1; solute carrier family 8 member 1.
Gene ID
20541
Accession Number
NP_035536.2
Reactivity
Mouse, Mus musculus
Target
Sodium/calcium exchanger 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SLC8A1 ELISA Kit (Mouse) (OKEH03086) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc8a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Slc8a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc8a1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
39.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
107 kDa
Specificity
Natural and recombinant Mouse Sodium/calcium exchanger 1
Protein Length
958
NCBI Gene Symbol
Slc8a1
Protein Name
P70414
Gene Name URL
Slc8a1
Nucleotide Accession Number
NM_011406.3

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