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GPX1 ELISA Kit (Mouse)

CAT: 0247-OKEH03081-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03081-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glutathione peroxidase 1
Gene Aliases
AI195024; AL033363; cellular glutathione peroxidase; CGP; CGPx; glutathione peroxidase 1; Gp; Gpx; GPx-; GPx-1; GSHPx; GSHPx-1; selenium-dependent glutathione peroxidase 1.
Gene ID
14775
Accession Number
NP_032186.2
Reactivity
Mouse, Mus musculus
Target
Glutathione peroxidase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GPX1 ELISA Kit (Mouse) (OKEH03081) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpx1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gpx1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpx1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
4.69-300U/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
Sensitivity
2.369U/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Notes
Formerly GWB-608VAM
Specificity
Natural and recombinant Mouse Glutathione peroxidase 1
Protein Length
201
NCBI Gene Symbol
Gpx1
Protein Name
P11352
Gene Name URL
Gpx1
Nucleotide Accession Number
NM_008160.6

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