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TMPRSS15 ELISA Kit (Human)

CAT: 0247-OKEH03076-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03076-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Transmembrane serine protease 15
Gene Aliases
Enterokinase; enterokinase catalytic subunit; enteropeptidase; ENTK; protease, serine, 7 (enterokinase) ; PRSS7; serine protease 7; Transmembrane protease serine 15; transmembrane protease, serine 15.
Gene ID
5651
Accession Number
NP_002763.2
Reactivity
Homo sapiens, Human
Target
Enteropeptidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TMPRSS15 ELISA Kit (Human) (OKEH03076) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMPRSS15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for TMPRSS15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TMPRSS15 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92.9%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
113 kDa
Specificity
Natural and recombinant Human Enteropeptidase
Protein Length
1019
NCBI Gene Symbol
TMPRSS15
Protein Name
P98073
Gene Name URL
TMPRSS15
Nucleotide Accession Number
NM_002772.2

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