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HEXA ELISA Kit (Rat)

CAT: 0247-OKEH03055-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03055-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Hexosaminidase subunit alpha
Gene Aliases
Beta-hexosaminidase subunit alpha; beta-N-acetylhexosaminidase subunit alpha; hexosaminidase A; hexosaminidase subunit A; Hexose aminidase A (alpha polypeptide) ; N-acetyl-beta-glucosaminidase subunit alpha.
Gene ID
300757
Accession Number
NP_001004443.1
Reactivity
Rat, Rattus norvegicus
Target
Beta-hexosaminidase subunit alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HEXA ELISA Kit (Rat) (OKEH03055) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hexa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hexa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hexa captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
61 kDa
Specificity
Natural and recombinant Rat Beta-hexosaminidase subunit alpha
Protein Length
528
NCBI Gene Symbol
Hexa
Protein Name
Q641X3
Gene Name URL
Hexa
Nucleotide Accession Number
NM_001004443.1

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