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GAR1 ELISA Kit (Human)

CAT: 0247-OKEH03050-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03050-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
GAR1 ribonucleoprotein
Gene Aliases
GAR1 homolog, ribonucleoprotein; GAR1 ribonucleoprotein homolog; H/ACA ribonucleoprotein complex subunit 1; NOLA1; nucleolar protein family A member 1; nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) ; snoRNP protein GAR1.
Gene ID
54433
Accession Number
NP_001129111.1
Reactivity
Homo sapiens, Human
Target
H/ACA ribonucleoprotein complex subunit 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology GAR1 ELISA Kit (Human) (OKEH03050) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human GAR1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GAR1 and incubated. The GAR1 found in the sample or standards competes with the biotinylated GAR1 for limited binding sites on the immobilized anti-human GAR1 antibody. Excess unbound biotinylated GAR1 and sample or standard GAR1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GAR1 captured in the well and inversely proportional to the amount of GAR1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94.9%
Sensitivity
0.404 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
140 kDa
Specificity
Natural and recombinant Human H/ACA ribonucleoprotein complex subunit 1
Protein Length
1251
NCBI Gene Symbol
GAR1
Protein Name
Q9NY12
Gene Name URL
GAR1
Nucleotide Accession Number
NM_001135639.1

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