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GIP ELISA Kit (Rat)

CAT: 0247-OKEH02992-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02992-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Gastric inhibitory polypeptide
Gene Aliases
Gastric inhibitory polypeptide; Glucose-dependent insulinotropic peptide; glucose-dependent insulinotropic polypeptide; Gludins; RATGLUDINS.
Gene ID
25040
Accession Number
NP_062604.1
Reactivity
Rat, Rattus norvegicus
Target
Gastric inhibitory polypeptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology GIP ELISA Kit (Rat) (OKEH02992) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Gip antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gip and incubated. The Gip found in the sample or standards competes with the biotinylated Gip for limited binding sites on the immobilized anti-rat Gip antibody. Excess unbound biotinylated Gip and sample or standard Gip is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gip captured in the well and inversely proportional to the amount of Gip which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 kDa
Specificity
Natural and recombinant Rat Gastric inhibitory polypeptide
Protein Length
144
NCBI Gene Symbol
Gip
Protein Name
Q06145
Gene Name URL
Gip
Nucleotide Accession Number
NM_019630.3

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