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SELP ELISA Kit (Rat)

CAT: 0247-OKEH02977-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02977-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Selectin P
Gene Aliases
CD62 antigen-like family member P; GMP-140; granule membrane protein 140; LECAM3; leukocyte-endothelial cell adhesion molecule 3; PADGEM; platelet activation dependent granule-external membrane protein; PSELECT; P-selectin; selectin, platelet.
Gene ID
25651
Accession Number
NP_037246.1
Reactivity
Rat, Rattus norvegicus
Target
P-selectin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SELP ELISA Kit (Rat) (OKEH02977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Selp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Selp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Selp captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Sensitivity
41.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
84 kDa
Specificity
Natural and recombinant Rat P-selectin
Protein Length
768
NCBI Gene Symbol
Selp
Protein Name
P98106
Gene Name URL
Selp
Nucleotide Accession Number
NM_013114.1

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