GH1 ELISA Kit (Mouse)

7732-18-5

Growth hormone

Gh1; Ghb1; Growth hormone; growth hormone b1; somatotropin.

14599

NP_032143.1

Mouse, Mus musculus

Somatotropin

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Aviva Systems Biology GH1 ELISA Kit (Mouse) (OKEH02967) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Gh1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gh1 and incubated. The Gh1 found in the sample or standards competes with the biotinylated Gh1 for limited binding sites on the immobilized anti-mouse Gh1 antibody. Excess unbound biotinylated Gh1 and sample or standard Gh1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gh1 captured in the well and inversely proportional to the amount of Gh1 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

0.156-10ng/mL

0.078 ng/mL

Store as indicated in product manual.

25 kDa

Natural and recombinant Mouse Somatotropin

216

Gh

P06880

Gh