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ZEB2 ELISA Kit (Human)

CAT: 0247-OKEH02951-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02951-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Zinc finger E-box binding homeobox 2
Gene Aliases
HSPC082; SIP1; SIP-1; SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; ZFHX1B; zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b.
Gene ID
9839
Accession Number
NP_001165124.1
Reactivity
Homo sapiens, Human
Target
Zinc finger E-box-binding homeobox 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ZEB2 ELISA Kit (Human) (OKEH02951) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ZEB2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZEB2 and incubated. The ZEB2 found in the sample or standards competes with the biotinylated ZEB2 for limited binding sites on the immobilized anti-human ZEB2 antibody. Excess unbound biotinylated ZEB2 and sample or standard ZEB2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZEB2 captured in the well and inversely proportional to the amount of ZEB2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.17 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
136 kDa
Specificity
Natural and recombinant Human Zinc finger E-box-binding homeobox 2
Protein Length
1214
NCBI Gene Symbol
ZEB2
Protein Name
O60315
Gene Name URL
ZEB2
Nucleotide Accession Number
NM_001171653.1

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