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Tnfsf13b ELISA Kit (Mouse)

CAT: 0247-OKEH02942-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02942-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tumor necrosis factor (ligand) superfamily, member 13b
Gene Aliases
B cell-activating factor; BAF; BAFF; B-cell-activating factor; BL; BLyS; D8Ertd387; D8Ertd387e; TAL; TALL1; TALL-1; THANK; TNFSF20; Tnlg7a; tumor necrosis factor ligand 7a; tumor necrosis factor ligand superfamily member 13B; zTNF; zTNF4.
Gene ID
24099
Accession Number
NP_296371.1
Reactivity
Mouse, Mus musculus
Target
Tumor necrosis factor ligand superfamily member 13B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tnfsf13b ELISA Kit (Mouse) (OKEH02942) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfsf13b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tnfsf13b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfsf13b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
34 kDa
Specificity
Natural and recombinant Mouse Tumor necrosis factor ligand superfamily member 13B
Protein Length
309
NCBI Gene Symbol
Tnfsf13b
Protein Name
Q9WU72
Gene Name URL
Tnfsf13b
Nucleotide Accession Number
NM_033622.1

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