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Tf ELISA Kit (Mouse)

CAT: 0247-OKEH02919-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02919-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Transferrin
Gene Aliases
AI266983; beta-1 metal-binding globulin; Cd176; H; HP; hpx; hypotransferrinemia with hemochromatosis; serotransferrin; siderophilin; Tf; Tfn.
Gene ID
22041
Accession Number
NP_598738.1
Reactivity
Mouse, Mus musculus
Target
Serotransferrin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tf ELISA Kit (Mouse) (OKEH02919) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tf captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
1.65 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
77 kDa
Specificity
Natural and recombinant Mouse Serotransferrin
Protein Length
697
NCBI Gene Symbol
Trf
Protein Name
Q921I1
Gene Name URL
Trf
Nucleotide Accession Number
NM_133977.2

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