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DIO2 ELISA Kit (Human)

CAT: 0247-OKEH02724-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02724-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Iodothyronine deiodinase 2
Gene Aliases
5DII; D2; deiodinase, iodothyronine type II; deiodinase-2; deiodonase-2; DIOII; selenoprotein Y; SELENOY; SelY; thyroxine deiodinase, type II; TXDI2; type 2 DI; type 2 iodothyronine deiodinase; type II iodothyronine deiodinase; type-II 5'deiodinase; type-II 5'-deiodinase.
Gene ID
1734
Accession Number
NP_000784.2
Reactivity
Homo sapiens, Human
Target
Type II iodothyronine deiodinase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology DIO2 ELISA Kit (Human) (OKEH02724) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human DIO2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DIO2 and incubated. The DIO2 found in the sample or standards competes with the biotinylated DIO2 for limited binding sites on the immobilized anti-Human DIO2 antibody. Excess unbound biotinylated DIO2 and sample or standard DIO2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DIO2 captured in the well and inversely proportional to the amount of DIO2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Human Type II iodothyronine deiodinase
Protein Length
273
NCBI Gene Symbol
DIO2
Protein Name
Q92813
Gene Name URL
DIO2
Nucleotide Accession Number
NM_000793.5

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