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Cd40 ELISA Kit (Mouse)

CAT: 0247-OKEH02680-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02680-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
CD40 antigen
Gene Aliases
AI326936; B-cell surface antigen CD40; Bp5; Bp50; CD40L receptor; GP39; HIGM1; IGM; IMD3; p5; p50; T-BAM; T-cell differentiation antigen; Tnfr; Tnfrsf5; TRAP; tumor necrosis factor receptor superfamily member 5.
Gene ID
21939
Accession Number
NP_035741.2
Reactivity
Mouse, Mus musculus
Target
Tumor necrosis factor receptor superfamily member 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cd40 ELISA Kit (Mouse) (OKEH02680) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd40 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cd40 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd40 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
7.96 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
32 kDa
Specificity
Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 5
Protein Length
289
NCBI Gene Symbol
Cd40
Protein Name
P27512
Gene Name URL
Cd40
Nucleotide Accession Number
NM_011611.2

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