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APOB ELISA Kit (Human)

CAT: 0247-OKEH02645-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02645-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Apolipoprotein B
Gene Aliases
ApoB-100; apoB-48; apolipoprotein B (including Ag (x) antigen) ; apolipoprotein B-100; apolipoprotein B48; FCHL2; FLDB; LDLCQ4.
Gene ID
338
Accession Number
NP_000375.2
Reactivity
Homo sapiens, Human
Target
Apolipoprotein B-100
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology APOB ELISA Kit (Human) (OKEH02645) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APOB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
20 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
516 kDa
Specificity
Natural and recombinant Human Apolipoprotein B-100
Protein Length
4563
NCBI Gene Symbol
APOB
Protein Name
P04114
Gene Name URL
APOB
Nucleotide Accession Number
NM_000384.2

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