Mannose ELISA Kit

• Gene Aliases:

  MN, Mannose, MN

• Reactivity:

  All Species

• Target:

  Mannose is a sugar monomer of the aldohexose series of carbohydrates. Mannose is a C-2 epimer of glucose. Mannose is important in human metabolism, especially in the glycosylation of certain proteins. Several congenital disorders of glycosylation are associated with mutations in enzymes involved in mannose metabolism.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology Mannose ELISA Kit (OKEH02595) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mannose antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Mannose and incubated. The Mannose found in the sample or standards competes with the biotinylated Mannose for limited binding sites on the immobilized anti-Mannose antibody. Excess unbound biotinylated Mannose and sample or standard Mannose is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Mannose captured in the well and inversely proportional to the amount of Mannose which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids

• Detection Range:

  1.56-100ng/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 93%

• Sensitivity:

  0.37 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant General Mannose

• Protein Name:

  Mannose

• CAS Number:

  7732-18-5