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Hydroxyproline ELISA Kit

CAT: 0247-OKEH02551-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02551-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Aliases
HYP, Hydroxyproline, HYP
Reactivity
All Species
Target
Hydroxyproline is a major component of the protein collagen. Hydroxyproline and proline play key roles for collagen stability. They permit the sharp twisting of the collagen helix. Hydroxyproline is found in few proteins other than collagen. For this reason, hydroxyproline content has been used as an indicator to determine collagen and/or gelatin amount. Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification) . The enzyme catalysed reaction takes place in the lumen of the endoplasmic reticulum. Although it is not directly incorporated into proteins, hydroxyproline comprises roughly 4% of all amino acids found in animal tissue, more than seven amino acids which are directly incorporated.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Hydroxyproline ELISA Kit (OKEH02551) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Hydroxyproline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hydroxyproline and incubated. The Hydroxyproline found in the sample or standards competes with the biotinylated Hydroxyproline for limited binding sites on the immobilized anti-Hydroxyproline antibody. Excess unbound biotinylated Hydroxyproline and sample or standard Hydroxyproline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hydroxyproline captured in the well and inversely proportional to the amount of Hydroxyproline which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Detection Range
62.5-4000nmol/L
Recovery
Mean recovery when spiking into Serum and Plasma = 97%
Sensitivity
31.25nmol/L
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant General Hydroxyproline
Protein Name
Hydroxyproline

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