Products for Research Use Only
RegisterLogin

Prostaglandin E2 ELISA Kit

CAT: 0247-OKEH02544-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH02544-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Aliases
PGE2, Prostaglandin E2, PGE2, dinoprostone
Reactivity
All Species
Target
The naturally occurring prostaglandin E2 (PGE2) is known in medicine as dinoprostone. It has important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts. PGE2 is also the prostaglandin that ultimately induces fever. Like other prostaglandins, dinoprostone can be used as an abortifacient. It is a direct vasodilator, relaxing smooth muscles, and it inhibits the release of noradrenaline from sympathetic nerve terminals. It does not inhibit platelet aggregation, where PGI2 does. It works by binding and activating the prostaglandin E2 receptor.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Prostaglandin E2 ELISA Kit (OKEH02544) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Prostaglandin E2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prostaglandin E2 and incubated. The Prostaglandin E2 found in the sample or standards competes with the biotinylated Prostaglandin E2 for limited binding sites on the immobilized anti-Prostaglandin E2 antibody. Excess unbound biotinylated Prostaglandin E2 and sample or standard Prostaglandin E2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prostaglandin E2 captured in the well and inversely proportional to the amount of Prostaglandin E2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 87%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant General Prostaglandin E2
Protein Name
Prostaglandin E2

Related Products

CatalogName
OAEG00203-100UGProstaglandin-E2 receptor EP2 Antibody
OAEG00204-100UGProstaglandin-E2 receptor EP3 Antibody
OAEG00208-100UGProstaglandin-E2 receptor EP2/EP3 Antibody
OKEH02618-96WProstaglandin F2alpha ELISA Kit
OKEH02602-96WProstaglandin D2 ELISA Kit
OKEH02602Prostaglandin D2 ELISA Kit (OKEH02602)
OKEH02618Prostaglandin F2alpha ELISA Kit (OKEH02618)
OKAG00655Cyclin E2 Colorimetric Cell-Based ELISA Kit (OKAG00655)
OPCA02148-20UGE2 Recombinant Protein
OAMA00220-1MGHCV E2 Antibody

Alternative Products

CatalogName
GTR10396915Porcine Prostaglandin ELISA
GTR10403383Bovine Prostaglandin ELISA
GTR10454085Human Prostaglandin ELISA
GTR10458379Mouse Prostaglandin ELISA
GTR10447139Sheep Prostaglandin ELISA
GTR10436012Rat Prostaglandin ELISA
MBS494345-01Prostaglandin E2 ELISA
GTR10429959Rabbit Prostaglandin ELISA
GTR10453996Chicken Prostaglandin ELISA
GTR10453740Monkey Prostaglandin ELISA

Popular Products