Products for Research Use Only
RegisterLogin

Estradiol ELISA Kit

CAT: 0247-OKEH02540-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH02540-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Aliases
E2, Estradiol, E2, oestradiol, 17beta-estradiol
Reactivity
All Species
Target
Estradiol (E2 or 17β-estradiol, also oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has 2 hydroxyl groups in its molecular structure. Estrone has 1 (E1) and estriol has 3 (E3) . Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its estrogenic effect. The serum levels of estradiol in males (14 - 55 pg/mL) are roughly comparable to those of postmenopausal women (< 35 pg/mL) . Estradiol in vivo is interconvertible with estrone; estradiol to estrone conversion being favored. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs, including the bones.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Estradiol ELISA Kit (OKEH02540) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Estradiol antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Estradiol and incubated. The Estradiol found in the sample or standards competes with the biotinylated Estradiol for limited binding sites on the immobilized anti-Estradiol antibody. Excess unbound biotinylated Estradiol and sample or standard Estradiol is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Estradiol captured in the well and inversely proportional to the amount of Estradiol which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 3.12 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant General Estradiol
Protein Name
Estradiol

Related Products

CatalogName
OKAU00066-5PLATEEstradiol Serum ELISA Kit
OKAU00066-1PLATEEstradiol Serum ELISA Kit
GWB-37E590-96WEstradiol ELISA
OAMA01459-1MGEstradiol Antibody
OOCD00162-200UGEstradiol (OVA - Conjugated)
OAEF00331-1MGEstradiol (E2) Antibody
OOCD00162-50UGEstradiol (OVA - Conjugated)
OOCD00161-10UGEstradiol (BSA - Conjugated)
OOCD00161-200UGEstradiol (BSA - Conjugated)
OOCD00162-10UGEstradiol (OVA - Conjugated)

Alternative Products

CatalogName
ES380SEstradiol ELISA
ECP7849Estradiol ELISA Kit
KET0003-01ELISA kit for Estradiol
RE5204117beta-Estradiol ELISA
MBS495068-01Estradiol (Rat) ELISA
MBS495045-01Estradiol (Sensitive) ELISA
EIA2693Estradiol ELISA [CE]
FR E-2000REstradiol ELISA (RUO)
SA E-6200Estradiol Saliva ELISA

Popular Products