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PGLYRP2 ELISA Kit (Human)

CAT: 0247-OKEH02439-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02439-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Peptidoglycan recognition protein 2
Gene Aliases
HMFT0141; N-acetylmuramoyl-L-alanine amidase; Peptidoglycan recognition protein 2; peptidoglycan recognition protein L; peptidoglycan recognition protein long; PGLYRPL; PGRPL; PGRP-L; tagL; tagL-alpha; tagl-beta; TAGL-like.
Gene ID
114770
Accession Number
NP_443122.3
Reactivity
Homo sapiens, Human
Target
N-acetylmuramoyl-L-alanine amidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology PGLYRP2 ELISA Kit (Human) (OKEH02439) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human PGLYRP2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PGLYRP2 and incubated. The PGLYRP2 found in the sample or standards competes with the biotinylated PGLYRP2 for limited binding sites on the immobilized anti-Human PGLYRP2 antibody. Excess unbound biotinylated PGLYRP2 and sample or standard PGLYRP2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PGLYRP2 captured in the well and inversely proportional to the amount of PGLYRP2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.054 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
62 kDa
Specificity
Natural and recombinant Human N-acetylmuramoyl-L-alanine amidase
Protein Length
576
NCBI Gene Symbol
PGLYRP2
Protein Name
Q96PD5
Gene Name URL
PGLYRP2
Nucleotide Accession Number
NM_052890.3

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