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GLO1 ELISA Kit (Human)

CAT: 0247-OKEH02424-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02424-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glyoxalase I
Gene Aliases
Aldoketomutase; epididymis secretory protein Li 74; GLOD1; glx I; GLYI; glyoxalase domain containing 1; Glyoxalase I; HEL-S-74; ketone-aldehyde mutase; lactoyl glutathione lyase; lactoylglutathione lyase; methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase.
Gene ID
2739
Accession Number
NP_006699.2
Reactivity
Homo sapiens, Human
Target
Lactoylglutathione lyase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GLO1 ELISA Kit (Human) (OKEH02424) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLO1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
32 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Human Lactoylglutathione lyase
Protein Length
184
NCBI Gene Symbol
GLO1
Protein Name
Q04760
Gene Name URL
GLO1
Nucleotide Accession Number
NM_006708.2

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