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MTR ELISA Kit (Human)

CAT: 0247-OKEH02417-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02417-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
5-methyltetrahydrofolate-homocysteine methyltransferase
Gene Aliases
5-methyltetrahydrofolate--homocysteine methyltransferase;5-methyltetrahydrofolate-homocysteine methyltransferase 1; cblG; cobalamin-dependent methionine synthase; HMAG; methionine synthase; MS; vitamin-B12 dependent methionine synthase.
Gene ID
4548
Accession Number
NP_000245.2
Reactivity
Homo sapiens, Human
Target
Methionine synthase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MTR ELISA Kit (Human) (OKEH02417) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human MTR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MTR and incubated. The MTR found in the sample or standards competes with the biotinylated MTR for limited binding sites on the immobilized anti-Human MTR antibody. Excess unbound biotinylated MTR and sample or standard MTR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MTR captured in the well and inversely proportional to the amount of MTR which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
141 kDa
Specificity
Natural and recombinant Human Methionine synthase
Protein Length
1265
NCBI Gene Symbol
MTR
Protein Name
Q99707
Gene Name URL
MTR
Nucleotide Accession Number
NM_000254.2

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