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ARID4B ELISA Kit (Human)

CAT: 0247-OKEH02367-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02367-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
AT-rich interaction domain 4B
Gene Aliases
180 kDa Sin3-associated polypeptide; ARID domain-containing protein 4B; AT rich interactive domain 4B (RBP1-like) ; AT-rich interactive domain-containing protein 4B; BCAA; BRCAA1; breast cancer-associated antigen 1; breast cancer-associated antigen BRCAA1; breast carcinoma-associated antigen; histone deacetylase complex subunit SAP180; Rb-binding protein homolog; RBBP1L1; RBP1L1; retinoblastoma-binding protein 1-like 1; SAP180; SIN3A-associated protein 180; sin3-associated polypeptide p180.
Gene ID
51742
Accession Number
NP_001193723.1
Reactivity
Homo sapiens, Human
Target
AT-rich interactive domain-containing protein 4B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ARID4B ELISA Kit (Human) (OKEH02367) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARID4B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARID4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARID4B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
148 kDa
Specificity
Natural and recombinant Human AT-rich interactive domain-containing protein 4B
Protein Length
1312
NCBI Gene Symbol
ARID4B
Protein Name
Q4LE39
Gene Name URL
ARID4B
Nucleotide Accession Number
NM_001206794.1

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