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LELP1 ELISA Kit (Human)

CAT: 0247-OKEH02310-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02310-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Late cornified envelope like proline rich 1
Gene Aliases
Late cornified envelope-like proline-rich protein 1; novel small proline rich protein; Novel small proline-rich protein.
Gene ID
149018
Accession Number
NP_001010857.1
Reactivity
Homo sapiens, Human
Target
Late cornified envelope-like proline-rich protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LELP1 ELISA Kit (Human) (OKEH02310) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LELP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LELP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LELP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
31 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Human Late cornified envelope-like proline-rich protein 1
Protein Length
98
NCBI Gene Symbol
LELP1
Protein Name
Q5T871
Gene Name URL
LELP1
Nucleotide Accession Number
NM_001010857.2

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