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LMX1B ELISA Kit (Human)

CAT: 0247-OKEH02309-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02309-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
LIM homeobox transcription factor 1 beta
Gene Aliases
FSGS10; LIM homeobox transcription factor 1-beta; LIM/homeobox protein 1.2; LIM/homeobox protein LMX1B; LMX1.2; LMX-1.2; NPS1.
Gene ID
4010
Accession Number
NP_001167618.1
Reactivity
Homo sapiens, Human
Target
LIM homeobox transcription factor 1-beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology LMX1B ELISA Kit (Human) (OKEH02309) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LMX1B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated LMX1B and incubated. The LMX1B found in the sample or standards competes with the biotinylated LMX1B for limited binding sites on the immobilized anti-Human LMX1B antibody. Excess unbound biotinylated LMX1B and sample or standard LMX1B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated LMX1B captured in the well and inversely proportional to the amount of LMX1B which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
0.13 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Human LIM homeobox transcription factor 1-beta
Protein Length
383
NCBI Gene Symbol
LMX1B
Protein Name
O60663
Gene Name URL
LMX1B
Nucleotide Accession Number
NM_001174147.1

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