TYROBP ELISA Kit (Human)

CAT: 0247-OKEH02258-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02258-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Transmembrane immune signaling adaptor TYROBP
Gene Aliases
DAP12; DNAX adaptor protein 12; DNAX-activation protein 12; KARAP; KAR-associated protein; killer-activating receptor-associated protein; PLOSL; PLOSL1; polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein.
Gene ID
7305
Accession Number
NP_001166985.1
Reactivity
Homo sapiens, Human
Target
TYRO protein tyrosine kinase-binding protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TYROBP ELISA Kit (Human) (OKEH02258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TYROBP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TYROBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TYROBP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Human TYRO protein tyrosine kinase-binding protein
Protein Length
113
NCBI Gene Symbol
TYROBP
Protein Name
O43914
Gene Name URL
TYROBP
Nucleotide Accession Number
NM_001173514.1

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