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AP1M1 ELISA Kit (Human)

CAT: 0247-OKEH02227-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02227-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Adaptor related protein complex 1 subunit mu 1
Gene Aliases
Adapter-related protein complex 1 subunit mu-1; adaptor protein complex AP-1 mu-1 subunit; adaptor protein complex AP-1 subunit mu-1; adaptor related protein complex 1 mu 1 subunit; Adaptor-related protein complex 1 subunit mu-1; AP-1 complex subunit mu-1; AP47; AP-mu chain family member mu1A; CLAPM2; clathrin adaptor protein AP47; clathrin assembly protein complex 1 medium chain 1; clathrin assembly protein complex 1 mu-1 medium chain 1; clathrin assembly protein complex 1, medium chain; clathrin assembly protein complex AP1, mu subunit; clathrin coat assembly protein AP47; clathrin coat-associated protein AP47; CLTNM; golgi adaptor AP-1 47 kDa protein; golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; mu1A; MU-1A; mu1A-adaptin; mu-adaptin 1.
Gene ID
8907
Accession Number
NP_001123996.1
Reactivity
Homo sapiens, Human
Target
AP-1 complex subunit mu-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology AP1M1 ELISA Kit (Human) (OKEH02227) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human AP1M1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AP1M1 and incubated. The AP1M1 found in the sample or standards competes with the biotinylated AP1M1 for limited binding sites on the immobilized anti-Human AP1M1 antibody. Excess unbound biotinylated AP1M1 and sample or standard AP1M1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AP1M1 captured in the well and inversely proportional to the amount of AP1M1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
49 kDa
Specificity
Natural and recombinant Human AP-1 complex subunit mu-1
Protein Length
423
NCBI Gene Symbol
AP1M1
Protein Name
Q9BXS5
Gene Name URL
AP1M1
Nucleotide Accession Number
NM_001130524.1

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