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PDE9A ELISA Kit (Human)

CAT: 0247-OKEH02199-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02199-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Phosphodiesterase 9A
Gene Aliases
CGMP-specific 3',5'-cyclic phosphodiesterase type 9; high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A; HSPDE9A2; phosphodiesterase PDE9A21.
Gene ID
5152
Accession Number
NP_001001567.1
Reactivity
Homo sapiens, Human
Target
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDE9A ELISA Kit (Human) (OKEH02199) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDE9A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDE9A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDE9A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.16 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
68 kDa
Specificity
Natural and recombinant Human High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Protein Length
593
NCBI Gene Symbol
PDE9A
Protein Name
O76083
Gene Name URL
PDE9A
Nucleotide Accession Number
NM_001001567.1

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