EEF1A1 ELISA Kit (Human)

CAT: 0247-OKEH02166-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02166-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Eukaryotic translation elongation factor 1 alpha 1
Gene Aliases
CCS3; CCS-3; cervical cancer suppressor 3; CTCL tumor antigen; EE1A1; EEF-1; EEF1A; eEF1A-1; EF1A; EF1a-like protein; EF-1-alpha-1; EF-Tu; elongation factor 1 alpha subunit; elongation factor 1-alpha 1; elongation factor Tu; epididymis secretory sperm binding protein; eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; LENG7; leukocyte receptor cluster (LRC) member 7; leukocyte receptor cluster member 7; prostate tumor-inducing protein 1; PTI1; translation elongation factor 1 alpha 1-like 14.
Gene ID
1915
Accession Number
NP_001393.1
Reactivity
Homo sapiens, Human
Target
Elongation factor 1-alpha 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EEF1A1 ELISA Kit (Human) (OKEH02166) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EEF1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EEF1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EEF1A1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.069 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Human Elongation factor 1-alpha 1
Protein Length
462
NCBI Gene Symbol
EEF1A1
Protein Name
P68104
Gene Name URL
EEF1A1
Nucleotide Accession Number
NM_001402.5

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