BCL6B ELISA Kit (Human)

CAT: 0247-OKEH02140-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02140-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
BCL6B transcription repressor
Gene Aliases
B cell CLL/lymphoma 6B; BAZF; B-cell CLL/lymphoma 6 member B protein; B-cell CLL/lymphoma 6, member B (zinc finger protein) ; bcl6-associated zinc finger protein; ZBTB28; zinc finger protein 62; ZNF62.
Gene ID
255877
Accession Number
NP_862827.1
Reactivity
Homo sapiens, Human
Target
B-cell CLL/lymphoma 6 member B protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BCL6B ELISA Kit (Human) (OKEH02140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL6B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL6B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL6B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.34 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 kDa
Specificity
Natural and recombinant Human B-cell CLL/lymphoma 6 member B protein
Protein Length
479
NCBI Gene Symbol
BCL6B
Protein Name
Q8N143
Gene Name URL
BCL6B
Nucleotide Accession Number
NM_181844.3

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