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RHOC ELISA Kit (Human)

CAT: 0247-OKEH02126-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02126-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Ras homolog family member C
Gene Aliases
ARH9; ARHC; epididymis secretory sperm binding protein; H9; oncogene RHO H9; ras homolog gene family, member C; RAS-related homolog 9; rho cDNA clone 9; rhoC GTPase; RHOH9; rho-related GTP-binding protein RhoC; small GTP binding protein RhoC.
Gene ID
389
Accession Number
NP_001036143.1
Reactivity
Homo sapiens, Human
Target
Rho-related GTP-binding protein RhoC
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RHOC ELISA Kit (Human) (OKEH02126) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RHOC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RHOC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RHOC captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Specificity
Natural and recombinant Human Rho-related GTP-binding protein RhoC
Protein Length
193
NCBI Gene Symbol
RHOC
Protein Name
P08134
Gene Name URL
RHOC
Nucleotide Accession Number
NM_001042678.1

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